RNAs and encoding the RGN polypeptide are each operably linked to a Wherein said nucleotide sequences encoding the one or more guide Nuclease (RGN) polypeptide comprising an amino acid sequence havingĪt least 95% sequence identity to SEQ ID NOs: 1, 11, 19, 27, 36,Ĥ5, or 54 or a nucleotide sequence encoding the RGN polypeptide Said target DNA sequence or one or more nucleotide sequencesĮncoding the one or more guide RNAs (gRNAs) and b) an RNA-guided A system for binding a target DNA sequence, said systemĬomprising: a) one or more guide RNAs capable of hybridizing to A cell comprising the nucleic acid molecule of claim 1.Ĩ. TracrRNA having at least 95% sequence identity to SEQ ID NOs: 3,ħ. The vector of claim 4, wherein the guide RNA comprises a Sequence having at least 95% sequence identity to SEQ ID NOs: 2,Ħ. The guide RNA comprises a CRISPR RNA comprising a CRISPR repeat Least one nucleotide sequence encoding said guide RNA, and wherein The vector of claim 4, wherein said vector further comprises at A vector comprising the nucleic acid molecule of claim 1.ĥ. ![]() Polypeptide is operably fused to a base-editing polypeptide.Ĥ. The nucleic acid molecule of claim 2, wherein the RGN Polypeptide is nuclease dead or functions as a nickase.ģ. The nucleic acid molecule of claim 1, wherein said RGN Promoter heterologous to said polynucleotide.Ģ. Polynucleotide encoding an RGN polypeptide is operably linked to a Of hybridizing to said target DNA sequence, and wherein said Sequence specific manner when bound to a guide RNA (gRNA) capable RGN polypeptide binds a target DNA sequence in an RNA-guided Identity to SEQ ID NOs: 1, 11, 19, 27, 36, 45, or 54 wherein said RNA-guided nuclease (RGN) polypeptide, wherein said polynucleotideĬomprises a nucleotide sequence encoding an RGN polypeptideĬomprising an amino acid sequence having at least 95% sequence A nucleic acid molecule comprising a polynucleotide encoding an The CRISPR system comprises an RNA-guided nuclease polypeptide and Further provided areĬRISPR systems for binding a target sequence of interest, wherein The nucleic acid molecules are also provided. Compositions comprise RNA-guided nuclease polypeptides,ĬRISPR RNAs, trans-activating CRISPR RNAs, guide RNAs, and nucleicĪcid molecules encoding the same. Sequence of interest, and modifying the expression of a sequence of Modifying a target sequence of interest, visualization of a target RNA-GUIDED NUCLEASES AND ACTIVE FRAGMENTS AND VARIANTS THEREOF ANDĬompositions and methods for binding to a target sequence of Bowen, Michael Coyle, Alexandra Briner Crawley, Tedd D. Invention is credited to Rodolphe Barrangou, Tyson D. The applicant listed for this patent is LifeEDIT, Inc. This patent application is currently assigned to LifeEDIT, Inc. patent application number 16/432321 was filed with the patent office on for rna-guided nucleases and active fragments and variants thereof and methods of use.
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